Novel enterokinase cleavage sequences are provided. Also disclosed are methods
for the rapid isolation of a protein of interest present in a fusion protein construct
including a novel enterokinase cleavage sequence of the present invention and a
ligand recognition sequence for capturing the fusion construct on a solid substrate.
Preferred embodiments of the present invention show rates of cleavage up to thirty
times that of the known enterokinase cleavage substrate (Asp)4-Lys-Ile.
