An improved method of nuclear transfer involving the transplantation of
donor differentiated cell nuclei into enucleated oocytes of the same
species as the donor cell is provided. The resultant nuclear transfer
units are useful for multiplication of genotypes and transgenic genotypes
by the production of fetuses and offspring, and for production of
isogenic CICM cells, including human isogenic embryonic or stem cells.
Production of genetically engineered or transgenic mammalian embryos,
fetuses and offspring is facilitated by the present method since the
differentiated cell source of the donor nuclei can be genetically
modified and clonally propagated.
