The invention relates to a method for detecting specific target-cells in a simple
and time saving way, using paramagnetic particles, antibodies recognizing the Fc
portions of target-cell associating antibodies and target-cell associating antibodies
directed to specific antigen determinants in the target-cell membranes. Incubation
of the cell suspension with a mild detergent and/or second set of antibodies or
antibody fragments, prelabeled or not with fluorescent agents, metallocolloids,
radioisotopes, biotincomplexes or certain enzymes allowing visualization, with
dramatically increase the specificity of the method. The method can further be
used for isolation of the target-cells by magnetic field application and kit for
performing the method according to the invention is described.
