Disclosed is a method for performing the steps of nucleic acid template
purification, a thermocycling reaction and purification of the products of the
thermocycling reaction characterised in that the steps take place sequentially
in a microfluidic disc. Also disclosed is a microstructure for fluids comprising
at least one inlet opening connected to a first chamber incorporating a means for
purifying template nucleic acid which, in turn, is connected to a second chamber
incorporating a means for a thermocycling reaction which, in turn, is connected
to a third chamber incorporating a means for purifying products of the thermocycling
reaction, and a microfluidic disc comprising a plurality of such microstructures.
