The invention provides purified and isolated DNA fragments from Bacillus anthracis
chromosomal DNA, primer sets and probes derived therefrom, as well as kits
and detection methods for B. anthracis. The methods of the invention provide
for specific detection of anthrax over closely related strains of Bacillus,
as well as accurate detection of low numbers of B. anthracis in an environmental
sample containing large amounts of non-specific DNA. The invention is applicable
to food, health care, and military applications.
