A method is provided for repopulating degenerated of immunetolerant mice
which lack mature B and T lymphocytes with xenogenic mammalian
hepatocytes, particularly primate hepatocytes to generate chimeric mice.
In addition, a method of generating a human hepatitis virus-infected
chimeric mouse is provided. A preferred xenogenic primate hepatocyte is
derived from human, chimpanzee or baboon. These chimeric mice are useful
in the investigation of host and viral mechanisms determining hepadnaviral
persistence and hepatocarcinogenesis. Methods for monitoring the
development of hepatitis and hepatocellular carcinoma as well as methods
for testing and screening anti-viral and anti-cancer compounds with this
model system are also provided.
Um método é fornecido repopulating degenerated dos ratos immunetolerant que faltam lymphocytes maduros de B e de T com hepatocytes mammalian xenogenic, particularmente hepatocytes do primata para gerar ratos chimeric. Além, um método de gerar hepatitis humano um rato chimeric vírus-virus-infected é fornecido. Um hepatocyte xenogenic preferido do primata é derivado do ser humano, do chimpanzé ou do babuíno. Estes ratos chimeric são úteis na investigação do anfitrião e de mecanismos viral que determinam o persistence e o hepatocarcinogenesis hepadnaviral. Os métodos para monitorar o desenvolvimento do hepatitis e do carcinoma hepatocelular assim como métodos para testar e selecionar compostos anti-viral e anti-cancer com este sistema modelo são fornecidos também.
