Single cell electroporation

   
   

A single cell electroporation assembly and method involve delivery of a substance into a single cell. The substance is combined into an electroporation fluid and placed into a container having a distal opening. The distal opening of the container is placed in proximity to a target cell. Electrical pulses are delivered between a first electrode which is at least partially disposed in the container, and a second electrode outside the cell, the cell being positioned between the distal opening and the second electrode. The electrical pulses induce temporary formation of pores in the cell membrane and the substance enters the cell through the pores by passive diffusion or by active electrophoretic motion.

Una asamblea y un método unicelulares del electroporation implican entrega de una sustancia en una célula. La sustancia se combina en un líquido del electroporation y se coloca en un envase que tiene una abertura distal. La abertura distal del envase se pone en proximidad a una célula de la blanco. Los pulsos eléctricos se entregan entre un primer electrodo que se disponga por lo menos parcialmente en el envase, y un segundo electrodo fuera de la célula, la célula que es colocada entre la abertura distal y el segundo electrodo. Los pulsos eléctricos inducen la formación temporal de poros en la membrana de la célula y la sustancia incorpora la célula a través de los poros por la difusión pasiva o por el movimiento electrophoretic activo.

 
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