Adjustable threshold switch

   
   

Provided are methods and compositions for regulating gene expression in a cell. The invention provides recombinant adjustable-threshold switches which contain a first inducible promoter-regulatory gene operon and a second constitutive promoter-regulatory gene operon. A threshold amount of activating agent is required to switch the inducible promoter "on" and the constitutive promoter "off". An adjustable-threshold switch is useful to reversibly switch the expression of a gene of interest between a stable "on" state and stable "off" state in response to an activating agent at or above a threshold concentration.

Con tal que sean los métodos y las composiciones para la expresión de regulación del gene en una célula. La invención proporciona los interruptores recombinant del ajustable-umbral que contienen un primer operon promotor-regulador inducible del gene y un segundo operon promotor-regulador constitutivo del gene. Requieren a una cantidad del umbral de agente que activa cambiar "on" inducible del promotor y "off" constitutivo del promotor. Un interruptor del ajustable-umbral es útil para cambiar reversible la expresión de un gene del interés entre un estado estable de "on" y el estado estable de "off" en respuesta a un agente que activa en o sobre una concentración del umbral.

 
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