Method and kit for detecting resistance to antiviral drugs

   
   

An assay and kit for the detection of phenotypic resistance of a retrovirus to a reverse transcriptase inhibitor drug in a biological sample. The assay is based on the direct analysis of the susceptibility of retroviral reverse transcriptase to inhibition by a reverse transcriptase inhibitor drug. The enzymatic activity of the reverse transcriptase is determined by measuring the DNA product produced when an RNA template and a first complementary DNA primer from a suitable region of the encephalomyocarditis virus genome are incubated with a biological sample containing reverse transcriptase in the presence of the drug to which resistance is being determined.

Un análisis y un kit para la detección de la resistencia phenotypic de un retrovirus a una droga reversa del inhibidor del transcriptase en una muestra biológica. El análisis es basado en el análisis directo de la susceptibilidad del transcriptase reverso retroviral a la inhibición por una droga reversa del inhibidor del transcriptase. La actividad enzimática del transcriptase reverso es determinada midiendo el producto de la DNA producido cuando una plantilla del RNA y una primera cartilla complementaria de la DNA de una región conveniente del genoma del virus del encephalomyocarditis se incuban con una muestra biológica que contiene transcriptase reverso en la presencia de la droga a la cual se está determinando la resistencia.

 
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