Method of determining the nucleotide sequence of oligonucleotides and DNA molecules

   
   

The present invention relates to a novel method for analyzing nucleic acid sequences based on real-time detection of DNA polymerase-catalyzed incorporation of each of the four nucleotide bases, supplied individually and serially in a microfluidic system, to a reaction cell containing a template system comprising a DNA fragment of unknown sequence and an oligonucleotide primer. Incorporation of a nucleotide base into the template system can be detected by any of a variety of methods including but not limited to fluorescence and chemiluminescence detection. Alternatively, microcalorimetic detection of the heat generated by the incorporation of a nucleotide into the extending template system using thermopile, thermistor and refractive index measurements can be used to detect extension reactions.

 
Web www.patentalert.com

< Electronic systems and component devices for macroscopic and microscopic molecular biological reactions, analyses and diagnostics

< Prognostic classification of endometrial cancer

> Methods of analyzing polymers using a spatial network of fluorophores and fluorescence resonance energy transfer

> Method for in vitro diagnosis of endometriosis

~ 00127