In this application is described substrates for high-throughput assays of
clostridial neurotoxin proteolytic activities. Two types of substrates are
described for use in assays for the proteolytic activities of clostridial
neurotoxins: (1) modified peptides or proteins that can serve as FRET
substrates and (2) modified peptides or proteins that can serve as
immobilized substrates. In both types a fluorescent molecules is present
in the substrate, eliminating the requirement for the addition of a
fluorigenic reagent. The assays described can be readily adapted for use
in automated or robotic systems.
