The present application relates to a method of making liposomes having
membrane proteins incorporated therein, the method comprising: providing
the membrane protein in solution; providing a solution of preformed
liposomes; and incubating the mixture. Prior to the step of providing a
solution of preformed liposomes, the liposomes are formed by combining a
mixture of phospholipids with a solution of at least one type of
unsaturated fatty acid. The methods of the present invention further
relate to the method of making a reagent comprising tissue factor
reconstituted into preformed liposomes. The method of the present
invention for making a tissue factor reagent comprises: providing tissue
factor in solution; providing a solution of preformed liposomes comprising
a mixture of phospholipids and at least one type of unsaturated fatty
acid; and incubating the mixture.
