Disclosed are systems and methods for identifying various tissue structure aspects, such as boundaries of arterial walls, within an image, such as an ultrasound image. Various measurements may be made using information with respect to the identified aspects of tissue structure. For example, intima-media thickness measurements may be made. Additionally or alternatively, tissue structure aspects, such as plaque within an artery, may be characterized, such as by determining a density thereof. Various information, such as measurement datums, used in identification of aspects of tissue structure in one image may be stored and applied to subsequent images, such as images of a video sequence.