An autofocus method can include moving a lens and reading out first image data associated with a plurality of image pixels during a first low light interval, modifying camera parameters to provide autofocus image light received during an autofocus light interval, acquiring second image data associated with the plurality of image pixels during the autofocus light interval, modifying camera parameters to substantially reduce image light received during a second low light interval, reading out the second image data during the second low light interval, and analyzing at least the first image data and the second image data to determine a focus position of the lens.