Primers have been isolated that are diagnostic for the detection of Vibrio harveyi. The primers are based on a portion of the Vibrio harveyi LuxR gene and may be used in primer directed amplification or nucleic acid hybridization assay methods.

 
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> Security hologram, method of recording same, and method of using same wherein the holographic imaging includes a single coherent beam of electromagnetic radiation in combination with a Lloyd's mirror to provide both reference and object beams

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