The present invention relates to a cosmetic composition comprising tissue-cultured adventitious roots itself of ginseng, and to a preparing method thereof. The cosmetic composition of the present invention is characterized in that effective ingredients are dip-extracted from the tissue cultured adventitious roots of a ginseng contained in the composition, without any additional process for preparing an extract of the tissue-cultured adventitious roots of a ginseng. In the cosmetic composition of the present invention, the effective ingredients are naturally and continuously dip-extracted from the tissue-cultured adventitious roots itself of a ginseng and maintained, and also allows a user to recognize visually the presence of the adventitious roots of a ginseng, thereby giving trust of the presence of the effective ingredient to the user, and improving user's satisfaction through its natural flavor. Further, the preparing method for the cosmetic composition is a simple and inexpensive process without any additional step, such as extraction, isolation and purification, and further has an advantage that an effective ingredient is dip-extracted from the adventitious roots itself of ginseng while not losing an effective ingredient.