The present invention relates to a novel tobacco cultivar designated AOB 176, which has low to intermediate nicotine content. The invention provides seeds of the cultivar AOB 176, plants and parts thereof of the cultivar AOB 176, a tissue culture derived from the cultivar AOB 176, hybrids produced from cultivar AOB 176 and lines derived from cultivar AOB 176, as well as genetically modified forms of the foregoing plants and tissue culture. Also provided are methods of producing cultivar AOB 176 plants, cultivar AOB 176 hybrid plants, and tobacco lines derived from cultivar AOB 176. In addition, products produced from the plants of the present invention are provided.