The present invention provides a method for replicating efficiently an RNA
containing fulllength HCV genomic sequence and a method for producing HCV
virus particles containing fulllength HCV replicon RNA or fulllength HCV
genomic RNA by using a cell culture system. Further, the present
invention relates to a method for producing hepatitis C virus particles
which comprises culturing a cell, into which a replicon RNA comprising a
nucleotide sequence comprising a fulllength genomic RNA sequence of
hepatitis C virus of the genotype 2a, at least one selectable marker gene
and/or at least one reporter gene and at least one IRES sequence or the
fulllength genomic RNA of hepatitis C virus of the genotype 2a is
introduced, and generating virus particles in the culture medium. Still
further the present invention relates also to a hepatitis C vaccine and
an antibody against hepatitis C virus particles.