A sensor of pyrophosphate which can detect pyrophosphate conveniently with high sensitivity in a method for measuring pyrophosphate in SNP typing utilizing a primer extension reaction is provided.A sensor of pyrophosphate which is characterized by including: an insulative substrate 1; an electrode system that is formed thereon and has a measurement electrode 2 and a counter electrode 3; and a plurality of reaction reagent layers that are provided on the substrate 1 and include pyrophosphatase, glyceraldehyde-3-phosphate dehydrogenase, diaphorase, glyceraldehyde-3-phosphate, oxidized nicotineamide adenine dinucleotide, an electronic mediator, a magnesium salt and a buffer component, reaction reagent layer 36 including the enzyme being separated from reaction reagent layer 35 including the buffer component, and reaction reagent layer 37 including glyceraldehyde-3-phosphate being separated from the reaction reagent layer 35 including the buffer component.

 
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< Method of selecting optimized SNP marker sets from multiple SNP markers associated with a complex disease

> PNA chip using plastic substrate coated with epoxy group-containing polymer, method of manufacturing the PNA chip, and method of detecting single nucleotide polymorphism using the PNA chip

> Polymorphisms in mitochondrial transcription factor A ("TFAM") gene and their associations with measures of marbling and subcutaneous fat depth in beef cattle

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