A device, method and kit for the electrophoretic separation and
purification of charged and neutral compounds in an analyte solution. The
device comprises a chamber (1), at least one wall of which is composed of
a chemical buffering system (4). A potential difference is applied across
the buffering system, resulting in the charged and neutral compounds
being differentially separated by extraction of the charged compounds
into the buffering system. The device also comprises means for collecting
the separated compounds, preferably in ampholyte-free or buffer-free
solution and optionally means for recycling the separated fractions.