Isolated polynucleotides comprising an S100B promoter are provided, where
an S100B regulatory element is operably joined to an S100B basal promoter
utilizing a non-native spacing between the promoter and regulatory
elements. The promoter may be operably linked to an expressible sequence,
e.g. reporter genes, genes encoding a polypeptide of interest, regulatory
RNA sequences such as miRNA, siRNA, anti-sense RNA, etc., and the like.
In some embodiments a cell comprising a stable integrant of an expression
vector is provided, which may be integrated in the genome of the cell.
The promoter may also be provided in a vector, for example in combination
with an expressible sequence. The polynucleotides find use in a method of
expressing a sequence of interest, e.g. for identifying or labeling
cells, monitoring or tracking the expression of cells, etc.