A method for separating and purifying nucleic acid, the method comprising:
(1) a step of contacting a sample solution containing nucleic acid with a
solid phase to adsorb the nucleic acid on the solid phase; (2) a step of
contacting a washing solution with the solid phase to wash the solid
phase in a state that the nucleic acid is adsorbed on the solid phase;
and (3) a step of contacting a recovering solution with the solid phase
to desorb the nucleic acid from the solid phase, wherein the sample
solution is prepared by including a step of removing a precipitate
component, and adding a surfactant and a water-soluble organic solvent to
a supernatant solution of the precipitate.