There is provided a method and/or system which allow on-chip
preconditioning of complex real-world samples and/or handling of limited
amounts of target material, and/or on-chip nucleic acid amplification
process, using a free droplet containing magnetic attractable material.
The nucleic acid amplification process comprises controlling the position
of the magnetic attractable material and performing the nucleic acid
amplification in a thermocycling droplet located onto at least one
temperature zone. The low thermal masses of the herein described
heaters/temperature sensors come along with fast temperature transitions
within the corresponding temperature zones allowing impressing
temperature gradients in at least one temperature zone between subsequent
or within the same thermocycle(s). Additionally, the variable residence
times of the droplet in a given temperature zone permit to customize the
denaturation, annealing and/or extension times within the same or between
different PCR runs. Additionally, the herein described method and/or
system allow amplification of one or more nucleic acid molecules.
Additionally, the herein described method and/or system allow real-time
monitoring with or without the presence of magnetic attractable material
bound to said nucleic acid molecule.