It is an object of the invention is to provide a light-emitting element in which failure of the light-emitting element due to separation can be controlled and stable luminescence can be obtained with high-efficiency and for a long stretch of time by controlling separation of layers constituting the light-emitting element. According to one aspect of a light-emitting element of the invention, the light-emitting element sandwiches a plurality of layers between a pair of electrodes, wherein at least one layer of the plurality of layers is a layer containing a substance selected from bismuth oxide, cobalt oxide, chromium oxide, copper oxide, nickel oxide, and titanium oxide, or at least one layer of layers different from a light-emitting layer among the plurality of layers is a mixed region of one substance selected from bismuth oxide, cobalt oxide, copper oxide, magnesium oxide, nickel oxide, zinc oxide, and titanium oxide and an organic compound.