Novel 2 polypeptides with binding affinity for the p185.sup.HER2 receptor,
designated heregulin 2-.alpha. and heregulin 2-.beta., have been
identified and purified from human tissue. The cDNA encoding the novel
heregulin 2-.alpha. has been isolated from human tissue and sequenced.
Provided herein is nucleic acid sequence of the heregulin 2-.alpha.
useful in the production of heregulin 2-.alpha. by recombinant means.
Further provided an amino acid sequence of heregulin 2-.alpha. and
heregulin 2-.beta.. Heregulins and their antibodies are useful as
therapeutic agents and in diagnostic methods.