An optical recording medium with a high productivity is provided which can certainly record/reproduce data even when a blue or blue-violet laser beam is used as an irradiation light. An optical recording medium has a substrate and a recording layer. The recording layer includes a recording portion and a coating portion. The recording portion and the coating portion are all made substantially from Bi and O. The ratio of the number of O atoms to the total number of Bi atoms and O atoms of the coating portion is less than that of the recording portion. The ratio of the number of O atoms of the recording portion is in the range of from 62% to 77%. The ratio of the number of O atoms to the total number of Bi atoms and O atoms of the coating portion is in the range of from 60% to 70%.

 
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> Protocol for detecting proteins in a culture containing an embryo

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