The present invention relates to a recombinant polynucleotide encoding a
polygene coding for at least three polypeptides wherein at least one of
the genes constituting the polygene is of non-viral origin, at least two
of the polypeptides encoded by the genes constituting the polygene are
each capable of at least transiently interacting with at least one other
polypeptide encoded by a gene of said polygene, and the genes
constituting the polygene are each connected to one mother by a sequence
coding for at least one protease cleavage site. The present invention
also relates to polyproteins encoded by the polygene. Further embodiments
of the present invention are a vector containing the recombinant
polypeptide, a host cell containing the recombinant polypeptide and/or
the vector and a non-human transgenic animal transformed with the
recombinant polypeptide and/or the vector. The present invention also
relates to methods for the production of the polynucleotide and for the
manufacture of multiprotein complexes. The embodiments of the present
invention are particularly useful in gene therapy, drug candidate
screening, vaccine production and crystallisation of multiprotein
complexes for structural investigations.