The present invention is directed to novel Neisseria meningitidis lipo-oligosaccharide inner core molecules, conjugates thereof and immunogenic compositions thereof. In particular embodiments, the invention relates to multivalent immunogenic compositions comprising five distinct Neisseria meningitidis inner core lipo-oligosaccharide epitope groups, wherein the multivalent compositions induce cross-reactive immune responses against Neisseria meningitidis lipo-oligosaccharide immunotypes.