One aspect of the invention provides a glucose monitor having a plurality
of tissue piercing elements, each tissue piercing element having a distal
opening, a proximal opening and interior space extending between the
distal and proximal openings; a sensing volume in fluid communication
with the proximal openings of the tissue piercing elements; sensing fluid
extending into the sensing volume; and a glucose sensor adapted to detect
a concentration of glucose in the sensing fluid within the sensing
volume. Another aspect of the invention provides A method of in vivo
monitoring of an individual's interstitial fluid glucose concentration
comprising: inserting distal ends of a plurality of tissue piercing
elements through a stratum corneum area of the individual's skin, the
tissue piercing elements each comprising a distal opening, a proximal
opening, and an interior space extending between the distal and proximal
opening; allowing interstitial fluid to flow into the interior space of
the tissue piercing elements to substantially fill the interior space;
filling substantially the entire interior space of the sensing area; and
sensing a glucose concentration of the sensing fluid.