A bicycle shift position control mechanism is configured to provide a bicycle shifting unit with multiple gear shifting in a single upshift operation or a single downshift operation. The shift position control mechanism that has a wire take up member, a positioning member, a first position maintaining member and a second position maintaining member. The positioning member rotates with the wire take up member between one of a plurality of predetermined shift positions. The first position maintaining member selectively moves between a first engagement position that holds the positioning member in one of the predetermined shift positions and a first disengagement position that releases the positioning member for rotational movement. The second position maintaining member selectively moes between a second engagement position that holds the positioning member in one of the predetermined shift positions and a second disengagement position that releases the positioning member for rotational movement.