The invention provides processes for generating transgenic plants or plant
cells transformed on their plastome, comprising introducing into plant
plastids a first and a second DNA molecule, wherein the first DNA
molecule contains a first region homologous to a region of the plastome
for directing plastome integration and a first sequence of interest, and
the second DNA molecule contains a second region homologous to a region
of the plastome and a second sequence of interest, whereby a sequence
segment of the first sequence of interest is homologous to a sequence
segment of the second sequence of interest, and selecting transformants
having an integration sequence stably integrated in the plastome, whereby
the integration sequence contains at least a portion of the first and
second sequences of interest as a continuous sequence. The invention
further provides plant cells, plants, and seeds of plants produced by
such processes.