A process for expression of a protein product in Aspergillus oryzae is
disclosed. The process comprises transforming Aspergillus oryzae with a
vector system comprising DNA-sequences encoding functions facilitating
gene expression, a suitable marker for selection of transformants, and a
DNA-sequence encoding the desired protein product. The process enables
industrial production of many different polypeptides and proteins in A.
oryzae. Examples of such products are chymosin or prochymosin other
rennets, proteases, lipases and amylases. Also disclosed is an effective
promoter for expression of a protein in Aspergillus. A preferred promoter
is the TAKA-amylase promoter or functional parts thereof. There is also
provided a process for the production of a recombinant Humicola lipase.
The recombinant Humicola lipase from A. oryzae differs from the native
lipase in having a greater glycosylation and in exhibiting an improved
thermostability.