A bicycle pedal assembly includes a pedal body having a sleeve tube and front and rear protruding portions. A front clamping member is mounted rotatably on the front protruding portion, and is biased by a front biasing member to rotate toward the sleeve tube. A locking member is connected to the front protruding portion, and is operable to abut against the front clamping member so as to lock the latter against rotational movement. A rear clamping member is mounted rotatably on the rear protruding portion, and is biased by a rear biasing member to rotate toward the sleeve tube. An adjusting member is connected to the rear clamping member, and is operable to adjust the biasing force of the rear biasing member.