The three-dimensional observation system of the present invention comprises an image picking-up part which has a parallax with respect to the object of observation and captures two images of the object of observation, an image display part which respectively displays the two images of the object of observation captured by the image picking-up part, and an optical system which is used to guide the two images of the object of observation displayed by the image display part to respective position in front of the left and right eyes of the observer facing the object of observation, such that the parallax direction of the image picking-up part and the parallax direction of the observer coincide, thus causing a three-dimensional image to be displayed.