The golf ball positioning dispenser for putting practice comprises a hollow tube for holding golf balls. A stop mechanism attached to a body block near the bottom end of the tube dispenses balls one at a time. The stop mechanism includes a pivotally attached biased lever that controls movement of a ball release pin and a ball retention pin into the tube in alternating cycles. The body block is attached to a travel wheel for circumferential travel around a practice hole. A telescoping radial arm is provided to keep travel of the travel wheel a predetermined user selected radial distance from the hole. Cams attached to the wheel engage the biased lever to dispense the golf balls from the tube at predetermined intervals of arc along a circumference of travel. A stand is included to stabilize the tube in an upright position.