A method for preparation of Timosaponin BII, which uses Chinese
traditional medicine Rhizoma Anemarrhenae or fresh rhizoma or fibrous
root of Anemarrhena asphodeloides Bge. as raw material, and comprises
isolation of Timosaponin BII by one or more processes selected from
solvent extraction, resin adsorption, polyamide chromatography, reversed
phase column chromatography, Sephadex LH-20 column chromatography, etc,
combining with conventional drying method such as reduced pressure
drying, freeze drying, spray drying, and so on. Timosaponin BII obtained
by the present method is of over 90% purity, and the method is simple,
practicable and suitable for industrial production.