A developing unit includes a plurality of developing rolls over which a developer is conveyed and supplied onto an image carrier which is rotatably supported; and at least one pair of the developing rolls being adjacent to each other. In the pair of the developing rolls, one developing roll rotates in a direction opposite to a rotary direction of the image carrier, and the other developing roll rotates in the same direction of the rotary direction of the image carrier. A regulation member is interposed between the pair of the developing rolls. The regulation member regulates a conveyed amount of the developer. The regulation member is disposed to be opposed to a magnet which is provided within the two adjacent developing rolls. A polarity of the magnet in the pair of developing rolls opposed to each other is different from each other.