Composition and method for simultaneously disinfecting and conditioning a wooden cutting surface, such as a cutting board or counter top. According to a preferred embodiment, the composition comprises a mixture of ethanol and food-grade mineral oil where the ethanol is present in an amount sufficient impart antiseptic properties. To the extent desired, other adjuvants, such as flavor enhancers, coloring agents, and the like may be added. The method comprises contacting the wooden cutting surface with such composition for a time sufficient to enable the ethanol to adequately disinfect the cutting surface. Preferably, such method may be performed by merely spraying or wiping the composition directly onto the wooden cutting surface.