The present invention relates generally to a method for the generation of
a substantially homogeneous population of undifferentiated cells. More
particularly, the present invention relates to the purification of a
substantially homogeneous population of stem cells and their progenitor
or precursor cells. Even more particularly, the present invention
provides a population of neural stem cells (NSCs). The subject invention
is particularly directed to NSCs and precursor cells with the capacity to
differentiate into cells and cell lineages required for the development,
maintenance or repair of the central nervous system in an animal such as
a mammal. The present invention is further directed to NSCs and
progenitor and/or precursor cells which are capable of proliferation and
differentiation into multiple cell lineages, such as but not limited to
neurons, oligodendrocytes, glia and astrocytes. The subject invention
further contemplates the use of NSCs and/or precursor cells for the
repair or regeneration of tissue, such as tissue associated with the
central nervous system, in an animal including a mammal. The NSCs of the
present invention may be used to identify naturally occurring molecules
such as cytokines as well as molecules obtained from natural product
screening or screening of chemical libraries which induce proliferation
of the NSCs. Such molecules are useful in the development of
therapeutics.