In a method for producing an L-amino acid by culturing a microorganism having an ability to produce an L-amino acid in a medium to produce and accumulate the L-amino acid in the medium and collecting the L-amino acid from the medium, a Gram-negative bacterium having the Entner-Doudoroff pathway and modified so that 6-phosphogluconate dehydratase activity or 2-keto-3-deoxy-6-phosphogluconate aldolase activity, or activities of the both are enhanced is used as the microorganism.

 
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< Method for in vitro molecular evolution of protein function

> DNA encoding favin-adenine-dinucleotide-dependent-D-erythronate-4-phosphate-de-hydroge- nase, pdxR, and microbial production of vitamin B.sub.6

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