Unique fusion genes are disclosed which are useful for transforming a wide
range of plants, and when used in tandem, result in a significant
alteration of the plant phenotype with respect to tolerance to the stress
from prolonged storage under either dark or cold conditions, or a
combination of cold and dark conditions. With intact plants such as
transplants, plants harboring these genes maintain the ability of recover
and grow normally after returned to normal growth conditions. With
isolated plant parts, such as cut flowers or foliage or fruits &
vegetables, leaf tissue maintains color quality and cells maintain
structural integrity during prolonged storage. Since the transgenes are
only activated in response to cold temperature, normal plant growth,
development, and function is not affected. The gene constructs include
(1) a cold-regulated gene (COR15a) promoter to drive an ipt coding
sequence that expresses IPT in the tissues of plants or plant parts
exposed to a short cold induction period; and (2) a cold-regulated gene
(COR15a) promoter to drive a (FAD7) coding sequence that expresses a
fatty acid desaturase enzyme in the tissues of plants or plant parts
exposed to a short cold induction period. Exemplary transformations
include chrysanthemum, tobacco, flowering tobacco, and petunia. Double
transgenic tobacco, and flowering tobacco both exhibited increased
survival under prolonged cold and dark storage conditions.