Apparatus for cell culture occupying little space, providing economical efficiency, reducing working labor, faulty manipulation, and promoting the prevention of contamination, which is suitable for small scale cell culture . The apparatus permits the execution of at least a medium replacement process and a passage culture process, and includes an aspirator unit for drawing a liquid, a chemical liquid supply unit for supplying a medium, a liquid for detachment, a washing liquid, a pipetting unit for quantitatively aspirating and discharging the liquid, a centrifugal unit for separating cells from the cell suspension in a centrifuge tube, and a handling unit for moving a culture vessel and the centrifuge tube to each unit. All units being constructed for storage in a clean bench. The apparatus may include a clean bench having a sterile operation space, and the aforementioned units may be removably stored within the sterile operation space.