A single pass simulated moving bed apparatus and methods are described for
continuously separating a target molecule from a liquid mixture, using a
simulated moving bed system. The simulated moving bed system includes a
plurality of filter cartridge modules in serial fluid communication. Each
filter cartridge module includes a volume of stationary phase
particulates adjacent a porous substrate layer. The volume of stationary
phase particulates has a bed height of less than 1 centimeter.