The present invention provides a pancreas induced in vitro that is useful
for developmental engineering and organ engineering, and a method wherein
a pancreas induced in vitro which contributes to the development of
diagnosis and treatment of pancreatic disorders for higher animals, can
artificially and efficiently be induced from a gastrula apart from the
presumptive region of pancreas. An explant which has a secretory
gland-like structure wherein several cells are gathered and which
expresses insulin is formed in vitro by treating the blastopore upper lip
of an early gastrula of a vertebrate such as Xenopus with retinoic acid
in vitro, and then culturing. The treatment with retinoic acid can be
carried out, for example, by treating with retinoic acid at a
concentration of 10.sup.-5 M or above for three hours.