The present invention provides a separating agent for optical isomers having a porous monolithic inorganic type carrier and polysaccharide or a derivative thereof supported on the monolithic inorganic type carrier, and a separation column for optical isomers in which the separating agent for optical isomers is held in a column tube. According to the invention, the separating agent for optical isomers and the separation column for optical isomers which have high asymmetry recognition ability and can be used particularly at a high flow rate when used for the separation of optical isomers is provided.