A method is described for the investigation of cytosine methylation in DNA
sequences. Triplex-forming oligomers are utilized, which preferably form
triplex structures at positions where cytosine unmethylated at position 5
is present. The triplexes block the transcription, replication and
amplification of the DNA. In particular, peptide nucleic acid oligomers
with modified nucleobases can be used as triplex-forming oligomers.