In a method for calibrating a biosensor for the amperometric determination
of creatinine in biological liquids, consisting of one electrode system
each for measuring the concentrations of creatinine and creatine, with at
least two calibration solutions being used, the electrode system for
measuring the creatinine concentration is calibrated with a creatinine
solution which, prior to the calibration measurement, was mixed in the
form of an acidic solution with an alkaline buffer solution, and the
electrode system for measuring the creatine concentration is calibrated
with a solution in which creatinine and creatine are at a thermodynamic
equilibrium.