A pulse jitter reduction circuit employs a low jitter system clock coupled to synchronize a pulse generating device and an ultra low jitter flip-flop to generate substantially jitter-free trigger signals employed to generate high voltage pulses for a flight tube of a time-of-flight mass spectrometer. By eliminating time fluctuations due to jitter in the triggering signal, the predictability of the arrival time of ions along a flight tube of a time-of-flight mass spectrometer is greatly improved, thereby improving the resolution of the mass spectrometer.

 
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