Replication-competent adenoviral vectors comprising an EBV-specific
transcriptional regulatory element (TRE) operably linked to a gene
required for adenovirus replication are provided. By providing for
transcriptional initiating regulation dependent upon transcription
factors that are only active in specific, limited cell types, virus
replication can be restricted to particular target cells. The modified
adenovirus may be used as a vehicle for introducing new genetic
capability, particularly associated with cytotoxicity for treating
neoplasia.