The present invention provides a straightforward and robust synthetic process for producing a chromatographic column with eluent-sensitive light diffracting properties based on an inherent photonic band structure and a chromatographic device using the chromatographic column. The present invention provides chromatographic devices employing a chromatographic column which in one embodiment is a photonic colloidal crystal which includes an assembly of colloidal microspheres assembled into a highly ordered array within a housing such as a tube with the highly ordered array being a photonic crystal along the length of the crystal, and a second embodiment which is an inverse construct of the first embodiment, where solid microspheres making up the photonic colloidal crystal chromatographic column are replaced with spherical voids or void spaces subsequent to infiltration of a material of selected refractive index. The photonic band structures of the first type of column made with colloidal particles and the second type of column made by inverting the first type of column may include a photonic band gap, a fundamental stop-band, higher stop-bands, or combinations thereof.

 
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